Blar i forfatter "Lin, Julie Qiaojin"

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    • On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons 

      Shigeoka, Toshiaki; Koppers, Max; Wong, Hovy Ho Wai; Lin, Julie Qiaojin; Cagnetta, Roberta; Dwivedy, Asha; de Freitas Nascimento, Janaina; van Tartwijk, Francesca W.; Ströhl, Florian; Cioni, Jean Michel; Schaeffer, Julia; Carrington, Mark; Kaminski, Clemens F.; Jung, Hosung; Harris, William A.; Holt, Christine E. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-12-10)
      Ribosome assembly occurs mainly in the nucleolus, yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ribosomes in the axon. We show that axonal RP translation is regulated through a sequence motif, ...

    • A Protocol for Single-Molecule Translation Imaging in Xenopus Retinal Ganglion Cells 

      Ströhl, Florian; Lin, Julie Qiaojin; Tartwijk, Francesca W. van; Wong, Hovy Ho Wai; Holt, Christine E.; Kaminski, Clemens F. (Chapter; Bokkapittel, 2020-04-29)
      Single-molecule translation imaging (SMTI) is a straightforward technique for the direct quantification of local protein synthesis. The protein of interest is fused to a fast-folding and fast-bleaching fluorescent protein, allowing one to monitor the appearance of individual fluorescence events after photobleaching of pre-existing proteins in the cell under investigation. The translation of individual ...